Hollow fiber micro-extraction technology and chromatographic analysis methods have achieved series progress

Hollow fiber micro-extraction technology and chromatographic analysis methods have achieved series progress

Pretreatment of complex samples has always been the focus of analytical chemistry research. The efficient, rapid, parallel, environment-friendly and miniaturized sample pretreatment technology has become an important research field in modern analytical chemistry. Hollow fiber tube micro-extraction technology (HFME) integrates sampling, extraction, and concentration. It has a small amount of organic solvent, is environment-friendly, has a low cost, and is simple to install. It is easy to use with modern chromatographic analyzers such as HPLC and GC. Widely used in food safety, drug evaluation, environmental monitoring and other livelihood areas.

Under the support of the National Natural Science Foundation of China, the team led by Shi Yanping, a researcher at the Northwest Key Laboratory of Plant Resources Chemistry of the Chinese Academy of Sciences Lanzhou Institute of Chemical Physics, has carried out Hollow Fiber Microextraction technology combined with chromatographic analysis since 2008. Based on systematic research, a variety of hollow fiber micro-extraction technologies have been developed and applied to the monitoring of contaminants and pesticide residues in foods and pharmaceuticals, and the monitoring of environmental pollutants.

First, by exploiting the advantages of porous hollow fibers, the development of hollow fiber liquid phase micro-extraction (HF-LPME) using porous polypropylene hollow fiber (PPHF) [Acta Chromatographica, 2010, 22 : 307; Biomedical Chromatography, 2010, 24: 1350; Journal of Chromatography B, 2010, 878: 2811].

Second, they overcome the shortcomings of hollow fiber liquid phase microextraction and use porous polypropylene hollow fiber as a template to prepare and establish hollow metal oxides hollow fiber sorptive micro-extraction (HF-SME). ), and combined with gas chromatography (GC-MS) analysis of trace pesticide residues in illicit additives melamine, fresh grapes and rhubarb in dairy products and organic pollutants in environmental water samples [Journal of Chromatography A, 2009 , 1216: 5467; Analytica Chimica Acta, 2009, 651: 182; Analytical and Bioanalytical Chemistry, 2010, 398: 1501; Food Chemistry, 2011, 127: 784].

Third, the advantages of carbon nanotubes strong adsorption (CNTs) and hollow fiber pore sieve filtration were fully exploited. With the porous polypropylene hollow fiber as the matrix, carbon nanotubes reinforced hollow fiber micro-extraction technology was developed and established. The solid phase micro-extraction (CNTs-HF-SPME) and the combination of high-performance liquid chromatography (HPLC) have been applied to the analysis of non-steroidal estrogens in milk and the residual non-stilbestrol in water samples. Anti-inflammatory drugs piroxicam and diclofenac sodium, broad-spectrum insecticide carbamate commonly used in apples, naphthalene acetic acid and naphthalene acetic acid as plant hormones in tomato, strychnine and strychnine, toxic alkaloids in Chinese urine [Talanta, 2012, 97: 222; Talanta, 2012, 100: 153; Talanta, 2013, 116: 188; Food Chemistry, 2013, 139: 246; Journal of Chromatography A, 2014, 1374: 23]. Fourth, taking full advantage of the advantages of ionic liquids and electrical membrane extraction, ionic liquid based electromembrane extraction (IL-EME) using hollow fibers as the support liquid membrane, combined with HPLC to efficiently detect urine in traditional Chinese medicine The toxic ingredients brucine and strychnine, pesticide residues in livestock tissues, etc. [Journal of Chromatography A, 2014, 1352: 1; RSC Advance, 2015, 5:37682].

Based on years of research on hollow fiber microextraction technology, the research group systematically reviewed the "Research Progress of Sample Preparation Technology for Hollow Fiber Tubes Modified by Various Adsorbents Functionally Modified" and published in the recently published TrAC magazine [Trends]. In Analytical Chemistry, 2015, 64: 109].

The above advanced analysis methods and detection technologies provide a scientific basis for food safety, drug evaluation, and environmental monitoring in China, and are conducive to promoting the improvement of China's food and drug safety and environmental protection levels.

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